Phoenix™ Hot Start Taq DNA Polymerase is a recombinant, thermostabile Taq DNA polymerase complexed with a thermolabile, neutralizing antibody that blocks the 5′→3′ polymerase activity prior to the initial DNA denaturation step of PCR (1,2). Such antibody-mediated Hot-Start capability enhances the overall specificity, sensitivity and yield of the PCR by reducing nonspecific amplification and primer-dimer formation prior to PCR cycling, and allows the convenience of reaction set up at room temperature. When the temperature of the PCR reaction mix reaches ≥94°C during the initial DNA denaturing step of PCR cycling, activity of the Taq DNA polymerase is fully restored. Phoenix Hot Start Taq DNA Polymerase, like standard Taq DNA polymerase, also has 5′→3′ exonuclease activity, but lacks any detectable 3′→5′ exonuclease activity.

Source of ProteinA recombinant E. coli strain carrying the Taq DNA polymerase gene from the thermophilic organism Thermus Aquaticus YT-1 complexed with a monoclonal antibody derived from murine cell culture.

Supplied in20 mM Tris-HCl100 mM NaCl0.1 mM EDTAStabilizer50% GlycerolpH 7.5 @ 25°C

Supplied With:5X Phoenix Hot Start Taq Reaction Buffer (B7590)5X Phoenix Hot Start Taq GC Reaction Buffer (B7591)

Unit Definition1 unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C.