φ29 DNA Polymerase responsible for the replication of the Bacillus Subtilis phage φ29 (1). The enzyme is a highly processive DNA polymerase (up to 70,000 base insertions per binding event) with a powerful strand displacement activity (2) and a 3′→ 5′ proofreading exonuclease function (3).
Source of ProteinA recombinant E. coli strain carrying the φ29 DNA Polymerase gene from bacteriophage φ29.
Supplied in 10 mM Tris-HCl100 mM KCl0.1 mM EDTA1 mM DTT0.5% Tween-200.5% NP-4050% glycerolpH 7.4 @ 25°C
Supplied WithB7020 (10X φ29 DNA Polymerase Reaction Buffer)
10X φ29 DNA Polymerase Buffer (B7020)500 mM Tris-HCl100 mM (NH4)2SO440 mM DTT100 mM MgCl2pH 7.5 @ 25°C
Unit Definition1 unit is defined as the amount of polymerase required to convert 0.5 pmol of dTTP into acid insoluble materialin 10 minutes at 30°C.
