酶/Taq DNA连接酶/20000 U/L6060L
商品编号:
L6060L
品牌:
Enzymatics inc
市场价:
¥7420.00
美元价:
3710.00
产品分类:
测序酶
公司分类:
Sequencing_enzyme
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
Taq DNA Ligase catalyzes the formation of a phosphodiester bond in duplex DNA containing adjacent 5′-phosphoryl and 3′-hydroxyl termini, using NAD+ as a cofactor.
Source of ProteinA recombinant E. coli strain carrying the cloned Taq DNA Ligase gene.
Supplied in10 mM Tris-HCl50 mM KCl1 mM DTT0.1 mM EDTA0.1% Tween-2050% glycerolpH 7.5 @ 25°C
Supplied With B6060 (10X Taq DNA Ligase Buffer)
10X Taq DNA Ligase Buffer (B6060)200 mM Tris-HCI250 mM KCl100 mM MgCl25 mM NAD0.1% Triton X-100pH 7.6 @ 25°C
Unit Definition1 unit is defined as the amount of Taq DNA Ligase required to join 50% of 1 µg of the 12-base cohesive ends of Lambda DNA cut with Sma I and Sal I in 50 µl 1X Taq DNA Ligase Buffer following a 10 minute incubation at 45°C.
品牌介绍
Enzymatics--核酸25 mM 100µmol dNTP Solution Mix--N2050L,N2050F
核酸25mM100µmoldNTPSolutionMix
摘要:四种天然脱氧核苷酸的等摩尔混合溶液(25mM)
产品描述
Anequimolar(25mM)mixtureofthefournaturaldeoxynucleotides.
四种天然脱氧核苷酸的等摩尔混合溶液(25mM)
SuppliedAs:25mMeachdNTP(dATP,dCTP,dGTP,dTTP)atpH7.5
产品信息
PartNumber N2050L,N2050F
Concentration 25mM
UnitSize 100µmol,20µmol
产品特性
StorageTemperature -25to-15°C
Purity ≥99%
BasePurity ≥99.5%
Pyrophosphate ≤0.003pmol/pmolofnucleotide
EnzScript™(MMLV逆转录酶,RNaseH-)
摘要:基于RNA的DNA聚合酶,无核酸内切酶活性
产品描述:
EnzScript™(M-MLV逆转录酶RNaseHminus)属于RNA依赖的DNA聚合酶,该酶无RNaseH活性。EnzScript™被用于从polyAmRNA或总RNA中产生首链cDNA以用于下游的一些用途,如RT-PCR,cDNA克隆或RNA-Seq的文库构建。RNaseH结构域中的点突变能增加酶的热稳定性,相比于野生型M-MLV逆转录酶,它支持全长转录物种产生更高的cDNA产量
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