E.coli RNase H (rnh) is an endoribonuclease which degrades the RNA strand of RNA/DNA hybrid molecules. RNase H digestion produces ribonucleotide molecules with 5-phosphate and 3-hydroxyl termini. RNAse H is nearly inactive against single or double-stranded RNA molecules.
Source of ProteinA recombinant E. coli strain carrying the RNAse H (rnh) gene from E.coli.
Supplied in20 mM Tris-HCl100 mM KCl10 mM MgCl20.1 mM EDTA0.1 mM DTT50% glycerolpH 7.9 @ 25°C
Supplied With B9220 (10X RNAse H Buffer)
10X RNAse H Buffer (B9220):500 mM Tris-HCl750 mM KCl30 mM MgCl2100 mM DTTpH 8.3 @ 25°C
Unit Definition1 unit is defined as the amount of enzyme that will hydrolyze 1 nmol of RNA from an 3H-labeled DNA:RNA hybrid molecule into acid-soluble material in 20 minutes at 37°C.
