Quality Control Analysis

HiFi PCR Master Mix Functional Assay: Quality of the 2X HiFi PCR Master Mix is tested functionally by amplification of a DNA library prepared from mixed bacterial genomic DNA with GC-content of 10-80%. The differences in library yield and profile among different lots must not exceed 15%. Sequencing of the amplified library must yield mapped reads >90% and normalized coverage between 0.7 and 1.3 across the full GC spectrum.

Enzymatics--核酸25 mM 100µmol dNTP Solution Mix--N2050L,N2050F 核酸25mM100µmoldNTPSolutionMix 摘要：四种天然脱氧核苷酸的等摩尔混合溶液(25mM) 产品描述 Anequimolar(25mM)mixtureofthefournaturaldeoxynucleotides. 四种天然脱氧核苷酸的等摩尔混合溶液(25mM) SuppliedAs:25mMeachdNTP(dATP,dCTP,dGTP,dTTP)atpH7.5 产品信息 PartNumber N2050L,N2050F Concentration 25mM UnitSize 100µmol,20µmol 产品特性 StorageTemperature -25to-15°C Purity ≥99% BasePurity ≥99.5% Pyrophosphate ≤0.003pmol/pmolofnucleotide EnzScript™(MMLV逆转录酶，RNaseH-) 摘要：基于RNA的DNA聚合酶，无核酸内切酶活性 产品描述： EnzScript™(M-MLV逆转录酶RNaseHminus)属于RNA依赖的DNA聚合酶，该酶无RNaseH活性。EnzScript™被用于从polyAmRNA或总RNA中产生首链cDNA以用于下游的一些用途，如RT-PCR,cDNA克隆或RNA-Seq的文库构建。RNaseH结构域中的点突变能增加酶的热稳定性，相比于野生型M-MLV逆转录酶，它支持全长转录物种产生更高的cDNA产量