Bst X DNA Polymerase is a thermostable DNA polymerase from Geobacillus, homologous to the DNA polymerase from Bacillus stearothermophilus (Bst). Bst X lacks 5’→3′ and 3’→5′ exonuclease activity and exhibits a stronger strand-displacement activity than Manta 1.0 DNA Polymerase that may allow it to perform better in isothermal amplification technologies.
Usage Notes:
- Optimal reaction temperature from 60-70°C.
- Heat inactivated at 80°C after 10 min incubation.
- High Tolerance to the non-ionic detergents Tween-20, Tween-80, TX-100, and NP-40 (up to 5% final concentration).
- Active in salt from 40 to 125 mM.
Buffer Description10x Bst X Xcelerator Reaction Buffer, contains 2 mM MgSO4 at 1X concentration. Depending on the application, it may be necessary to adjust the MgSO4 from 2-10 mM final concentration.
Source of ProteinA recombinant E.coli strain carrying the Bst X DNA Polymerase (exo-) polymerase gene.
Unit Definition1 unit is defined as the amount of polymerase required to convert 10 nmol of dNTPs into acid insoluble material in 30 minutes at 65°C
Molecular weight66,494 Daltons
