E.coli Endonuclease VIII functions as both an N-glycosylase (by excising oxidative base lesions) and an AP lyase (by subsequently cleaving the phosphodiester backbone), leaving terminal phosphates at the 5′ and 3′ ends. (1) Damaged bases removed by Endonuclease VIII include: urea, 5, 6- dihydroxythymine, thymine glycol, 5-hydroxy-5- methylhydanton, uracil glycol, 6-hydroxy-5, 6-dihydrothymine and methyltartronylurea (2,3).
Source of ProteinAn E. coli strain which carries the cloned Endonuclease VIII gene.
Supplied in10 mM Tris-HCl250 mM NaCl0.1 mM EDTA50% glycerolpH 8.0 @ 25°C
Supplied With:B9080 10X Endonulease Vlll Buffer
10X Endonuclease Vlll Buffer (B9080)100 mM Tris-HCl750 mM NaCl10 mM EDTApH 8.0 @ 25°C
Unit DefinitionOne unit is defined as the amount of enzyme required to cleave 1 pmol of an oligonucleotide duplex containing a single AP site in 1 hour at 37°C.
