The End-Repair Mix converts DNA containing damaged or incompatible 5′- and/or 3′-protruding ends to 5′-phosphorylated, blunt-ended DNA. This low-concentration formulation of the End-Repair Mix is compatible with applications requiring <1 microgram of DNA to be prepared for blunt-end ligation. The conversion to blunt-ended DNA is accomplished by exploiting the 5′→3′ polymerase and 3′→5′ exonuclease activities of T4 DNA Polymerase (P7080). T4 Polynucleotide Kinase (Y9040) ensures that the ends of the blunt-ended DNA fragments are 5′-phosphorylated for subsequent ligation by T4 DNA Ligase (L6030-HC).
Source of ProteinPurified from strains of E. coli that express the recombinant T4 DNA Polymerase, and T4 Polynucleotide Kinase genes, respectively.
Supplied in100 mM KCl10 mM Tris-HCl0.1 mM EDTA1 mM DTT0.1% Triton X-10050% glycerolpH 7.4 @ 25°C
Supplied WithB9140 (10X End-Repair Buffer)N2060 (1 mM dNTPs)
10X End-Repair Buffer (B9140):1 M Tris-HCl500 mM NaCl100 mM MgCl250 mM DTT0.25% Triton-X 100pH 7.5 @ 25°C
