Exonuclease III is a 3′→5′ exonuclease which acts by digesting one strand of a dsDNA duplex at a time or digesting the RNA strand of an RNA-DNA heteroduplex (1). Exonuclease III breaks phosphodiester bonds on the 5′ side of AP sites in both dsDNA and ssDNA (3), removes 3′ terminal groups on dsDNA (3), increases MutY turnover (4), and efficiently degrades 3′ recessed but not 3′ protruding DNA ends (creating 5′ overhangs) (5). Exo III removes a limited number of nucleotides per binding event, resulting in coordinated progressive deletions within the population of DNA molecules (1).
Source of ProteinPurified from a strain of E. coli that expresses the recombinant Exonuclease III gene.
Supplied in25 mM Tris-HCl50 mM KCl1.0 mM DTT0.1% mM EDTA50% GlycerolpH 8.0 @ 25°C
Supplied WithB0130 (10X Yellow Buffer)
10X Yellow Buffer (B0130)100 mM Bis-Tris-Propane-HCl100 mM MgCl210 mM DTTpH 7.0 @ 25°C
Unit DefinitionOne unit is defined as the amount of enzyme required to produce 1 nmol of acid-soluble total nucleotide in 30 minutes at 37°C.
