Uracil-DNA Glycosylase catalyzes the hydrolysis of the N-glycosylic bond between the uracil and sugar, leaving an abasic site in uracil-containing single or double-stranded DNA. The enzyme shows no measurable activity on short oligonucleotides (<6 bases), or RNA substrates.
Source of ProteinA recombinant E. coli strain carrying the Uracil DNA Glycosylase gene from E. coli K-12.
Supplied in10 mM Tris-HCl50 mM NaCl1 mM DTT0.1 mM EDTA50% glycerolpH 7.5 @ 25°C
Supplied WithB5010 (10X UDG Reaction Buffer)
10X UDG Reaction Buffer (B5010):200mM Tris-HCI10 mM DTT10 mM EDTApH 8.0 @ 25°C
Unit Definition1 unit is defined as the amount of enzyme that catalyzes the release of 1.8 nmol of Uracil in 30 minutes from double-stranded, tritiated, Uracil containing-DNA at 37°C in 1X UDG Reaction Buffer.
